Identification and characterization of a major QTL responsible for erect panicle trait in japonica rice (Oryza sativa L.)

Chang-Jie Yan, Ji-Hua Zhou, Song Yan, Feng Chen, Martin Yeboah, Shu-Zhu Tang, Guo-Hua Liang, Ming-Hong Gu
Theoretical and Applied Genetics, 2007, 115(8): 1093-1100  DOI: 10.1007/s00122-007-0635-9;      追溯原文......本站官方QQ群:62473826

Panicle erectness (PE) is one of the most important traits for high-yielding japonica cultivars. Although several cultivars with PE trait have been developed and released for commercial production in China, there is little information on the inheritance of PE traits in rice. In the present study, 69 widely cultivated japonica cultivars and a double haploid (DH) population derived from a cross between a PE cultivar (Wuyunjing 8) and a drooping panicle cultivar (Nongken 57) were utilized to elucidate the mechanisms of PE formation and to map PE associated genes. Our data suggested that panicle length (PL) and plant height (PH) significantly affected panicle curvature (PC), with shorter PL and PH resulting in smaller PC and consequently more erect. A putative major gene was identified on chromosome 9 by molecular markers and bulk segregant analysis in DH population. In order to finely map the major gene, all simple sequence repeats (SSR) markers on chromosome 9 as well as 100 newly developed sequence-tagged site (STS) markers were used to construct a linkage group for quantitative trait locus (QTL) mapping. A major QTL, qPE9-1, between STS marker H90 and SSR marker RM5652, was detected, and accounted for 41.72% of PC variation with pleiotropic effect on PH and PL. another QTL, qPE9-2, was also found to be adjacent to qPE9-1. In addition, we found that H90, the nearest marker to qPE9-1, used for genotyping 38 cultivars with extremely erect and drooping panicles, segregated in agreement with PC, suggesting the H90 product was possibly part of the qPE9-1 gene or closely related to it. These data demonstrated that H90 could be used for marker-aided selection for the PE trait in breeding and in the cloning of qPE9-1.


直立穗是高产粳稻品种最重要的性状之一。尽管在中国有很多的直立穗品种被育成并被商业推广,但是水稻直立穗性状的遗传研究还很少。本研究,利用69个广泛种植的粳稻品种和一个来源于直立穗品种(武育粳8号)与弯穗品种(农垦57)杂交的DH群体,来阐明直立穗形成的机制及直立穗基因的定位,数据显示,穗长和株高显著影响穗弯曲度,较短的穗长和较矮的株高导致较小的穗弯曲度,从而穗子更加直立。通过分子标记和混合分离分析法,在DH群体中,在第9染色体鉴定到一个主效基因。为了精细定位该主效基因,第9染色体的所有SSR标记和100个新发展的STS标记被用于该QTL遗传连锁群的构建。鉴定到一个主效QTL qPE9-1,位于STS标记H90和SSR标记RM5652之间,对穗弯曲度的表型贡献值为41.72%,并且对株高和穗长具有多效性。另外还定位到一个与qPE9-1毗邻的QTL,qPE9-2。此外,我们发现利用离qPE9-1最近的标记H90对38个穗型非常直立和弯曲的水稻品种进行基因型鉴定,该标记的分离与穗弯曲度的分离一致。表明H90可能是qPE9-1基因的基因内标记或者与该基因紧密连锁。上述数据表明H90能够用于直立穗性状的分子标记辅助选择和克隆qPE9-1

  G蛋白γ亚基; 直立型密穗基因; 直立穗基因 DEP1; DN1; qPE9-1; qNGR9