Deletion in a Quantitative Trait Gene qPE9-1 Associated With Panicle Erectness Improves Plant Architecture During Rice Domestication

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Yong Zhou, Jinyan Zhu, Zhengyi Li, Chuandeng Yi, Jun Liu, Honggen Zhang, Shuzhu Tang, Minghong Gu, Guohua Liang
Genetics, 2009, 183(1): 315-324  DOI: 10.1534/genetics.109.102681;      追溯原文......本站官方QQ群:62473826

Rice plant architecture is an important agronomic trait and a major determinant in high productivity. Panicle erectness is the preferred plant architecture in japonica rice, but the molecular mechanism underlying domestication of the erect panicle remains elusive. Here we report the map-based cloning of a major quantitative trait locus, qPE9-1, which plays an integral role in regulation of rice plant architecture including panicle erectness. The R6547 qPE9-1 gene encodes a 426-amino-acid protein, homologous to the keratin-associated protein 5-4 family. The gene is composed of three Von Willebrand factor type C domains, one transmembrane domain, and one 4-disulfide-core domain. Phenotypic comparisons of a set of near-isogenic lines and transgenic lines reveal that the functional allele (qPE9-1) results in drooping panicles, and the loss-of-function mutation (qpe9-1) leads to more erect panicles. In addition, the qPE9-1 locus regulates panicle and grain length, grain weight, and consequently grain yield. We propose that the panicle erectness trait resulted from a natural random loss-of-function mutation for the qPE9-1 gene and has subsequently been the target of artificial selection during japonica rice breeding.

与穗直立相关的数量性状基因qPE9-1的碱基缺失在水稻驯化过程中改良了株型

水稻株型是一个重要的农艺性状,对高产具有主要的决定作用。直立穗在粳稻育种中被广泛应用,然而直立穗驯化的分子机制依然未知。本研究,我们图位克隆了一个主效数量性状基因qPE9-1,其对水稻形态建成具有整体作用,包括对直立穗性状。来源于水稻品种R6547的qPE9-1基因,编码一个含有426个氨基酸的蛋白,是角蛋白5-4家族的成员。这个基因包括3个血管性血友病因子(vWF)C型结构域,1个跨膜结构域,1个4-硫化物-核心结构域。对一系列近等基因系和转基因系进行表型比较,结果显示功能等位基因(qPE9-1)导致弯曲穗,功能缺失突变等位基因(qpe9-1)导致直立穗。此外,qPE9-1基因还控制穗长、粒长、粒重,进而控制产量。我们推测直立穗性状来源于qPE9-1基因一个自然的随机的功能缺失突变,随后在粳稻育种中通过人工选择被固定下来。


基因列表
  G蛋白γ亚基; 直立型密穗基因; 直立穗基因 DEP1; DN1; qPE9-1; qNGR9